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An international team of researchers created nanodiamond sensors that can act as both heat sources and thermometers, and is using them to measure the thermal conductivity inside living cells, which may lead to new diagnostics tools and therapies for cancer.
A team of scientists from Osaka University, The University of Queensland, and the National University of Singapore's Faculty of Engineering used tiny nanodiamonds coated with a heat-releasing polymer to probe the thermal properties of cells. When irradiated with light from a laser, the sensors acted both as heaters and thermometers, allowing the thermal conductivity of the interior of a cell to be calculated. This work may lead to a new set of heat-based treatments for killing bacteria or cancer cells.
Even though the cell is the fundamental unit of all living organisms, some physical properties have remained difficult to study in vivo. For example, a cell's thermal conductivity, as well as the rate that heat can flow through an object if one side is hot while the other side is cold, remained mysterious. This gap in our knowledge is important for applications such as developing thermal therapies that target cancer cells, and for answering fundamental questions about cell operation.
(a) Illustration of the structure of a nanodiamond quantum sensor coated with a pyrogenic polymer, and how it operates as a hybrid nanoheater/thermometer. (b) Electron microscope image of hybrid sensors. (c) Working principle of the hybrid sensor for measuring nanometric thermal conductivity. In a medium with high thermal conductivity, the temperature increase of the diamond sensor is moderate, because heat readily diffuses away. In contrast, in a low thermal conductivity medium, the temperature rise is significantly larger. Intracellular thermal conductivity can be determined by measuring the temperature change of the hybrid sensors in cells.
Now, the team has developed a technique that can determine the thermal conductivity inside living cells with a spatial resolution of about 200 nm. They created tiny diamonds coated with a polymer, polydopamine, that emit both fluorescent light as well as heat when illuminated by a laser. Experiments showed that such particles are non-toxic and can be used in living cells. When inside a liquid or a cell, the heat raises the temperature of the nanodiamond.
In media with high thermal conductivities, the nanodiamond did not get very hot because heat escaped quickly, but in an environment of low thermal conductivity, the nanodiamonds became hotter. Crucially, the properties of the emitted light depend on the temperature, so the research team could calculate the rate of heat flow from the sensor to the surroundings.
(a) Temperature increases observed with hybrid sensors in air, water, oil, and inside cell. These results are consistent with the idea that higher temperature increases occur in solvents with smaller thermal conductivities. The literature values for the thermal conductivities of air, water, and oil are 0.026, 0.61, and 0.135 W/m* K, respectively. (b) Bright field microscopic image of a HeLa cell with a hybrid sensor inside.
Having good spatial resolution allowed measurements in different locations inside the cells. "We found that the rate of heat diffusion in cells, as measured by the hybrid nanosensors, was several times slower than in pure water, a fascinating result which still waits for a comprehensive theoretical explanation and was dependent on the location," senior author Taras Plakhotnik says.
"In addition to improving heat-based treatments for cancer, we think potential applications for this work will result in a better understanding of metabolic disorders, such as obesity," senior author Madoka Suzuki says. This tool may also be used for basic cell research, for example, to monitor biochemical reactions in real time.
Read the original article on Osaka University.