Standard Practice for Performing Cryo-Transmission Electron Microscopy of Liposomes

Standard Practice for Performing Cryo-Transmission Electron Microscopy of Liposomes

Standard Number ASTM E3143 - 18b
Organization American Society for Testing and Materials USA
Level National
Category Guide | Practice
Status
  • FEB 2019 Published
ABSTRACT
This practice covers procedures for vitrifying and recording images of a suspension of liposomes with a cryo-transmission electron microscope (cryo-TEM) for the purpose of evaluating their shape, size distribution and lamellarity for quality assessment. The sample is vitrified in liquid ethane onto specially prepared holey, ultra-thin, or continuous carbon TEM grids, and imaged in a cryo-holder placed in a cryo-TEM.