Nowadays, the three-dimensional hydrogel (3D hydrogel) is promising to be used in tissue engineering as cell scaffold. And femtosecond laser two-photon polymerization (TPP) as the emerged 3D printing technology has advantages in fabricating precise 3D hydrogel because of its 3D fabrication capability and high processing resolution.
However, it is difficult to fabricate the microstructures in the aqueous solution due to the poor initiating efficiency of photoinitiator and certain cytotoxicity. Therefore, the design and synthesis of water-soluble photoinitiators with large two-photon absorption (TPA) cross-sections are of great significance for expanding the application of TPP in hydrogel processing.
In order to break through the bottleneck of TPA and water solubility of the TPA material, Prof. ZHENG Meiling's team from TIPC has proposed the ionic molecular design idea and strategies to further improve the nonlinear optical property by host-guest chemistry. This work has been published in ACS Applied Material Interfaces.
In this study, the ionic carbazole water-soluble photoinitiator 3,6-Bis[2-(1-methyl-pyridinium)vinyl]-9-methyl-carbazole diiodide (BMVMC) was designed and synthesized to realize the 3D printing in aqueous phase. And the host-guest chemistry reaction was utilized to improve the TPP micro-environment while the hydrophilic cucurbit uril (CB7) and BMVMC were used as the host-guest molecules. The binding ratio of BMVMC and CB7 in the complex was demonstrated as 1:1 both theoretically and experimentally.
According to the researchers, the TPA cross section of the complex has been enhanced five times significantly. Thus, a relatively low laser threshold of 3.7 mW and the resolution of 180 nm were obtained in aqueous phase. Benefiting from the fluorescence property of the photoinitiator, confocal fluorescence images could be recorded without any assistance of fluorescence probes.
Schematic Illustration of the Green Environment-Friendly Micronano 3D printing of Biocompatible Hydrogel using the Ionic Water Soluble Two-photon Photoinitiator.
Subsequently, the biocompatible 3D engineered hydrogel scaffold microstructure with good mechanical property was achieved in aqueous phase. The proposed protocol would open up new avenues for the material design and prospective application in cell biology and tissue engineering.
Read the original article on Chinese Academy of Sciences (CAS).