Nanotechnology – CM-H2DCF-DA assay to assess nanoparticle-induced intracellular reactive oxygen species (ROS) production in macrophage cell line RAW 264.7
| Standard Number | DS/ISO/TS 19006:2016 |
|---|---|
| Organization |
Danish Standards
|
| Level | National |
| Category | Test Method | Characterization | Measurement |
| Status |
|
ABSTRACT
ISO/TS 19006:2016 describes how to test and evaluate results obtained from in vitro ROS generation in RAW 264.7 macrophage cells exposed to nano-objects, nanoparticles, their aggregates and agglomerates using the CM-H2DCFDA assay. The protocol in ISO/TS 19006:2016 is limited to use of a 24 well plate so if other plates were to be used, volumes would need to be adjusted and the protocol steps validated to ensure confidence in the test results.