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Leukocyte Proliferation Assay

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Standard Number ITA-6
Organization Nanotechnology Characterization Laboratory
Level National
Category Specification
Status
  • JAN 2009 Published
  • JAN 2020 Withdrawn
ABSTRACT
This document describes a protocol for assessing the effect of a nanoparticle formulation on the basic immunologic function of human lymphocytes, i.e. measurement of lymphocyte proliferative responses (1, 2). This assay will allow for measurement of a nanoparticles’ ability to induce proliferative response of human lymphocytes or to suppress that induced by phytohemaglutinin (PHA-M).