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LLC-PK1 Kidney Cell Apoptosis Assay

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Standard Number GTA-5
Organization Nanotechnology Characterization Laboratory
Level National
Category Specification
Status
  • JAN 2010 Published
ABSTRACT
This protocol describes the monitoring of nanoparticle treated porcine renal proximal tubule cells (LLC-PK1) for apoptosis, as part of the in vitro NCL preclinical characterization cascade. The protocol utilizes a fluorescent method to determine the degree of caspase-3 activation.