Leukocyte Proliferation Assay

Standard Number ITA-6.3
Organization Nanotechnology Characterization Laboratory
Level National
Category Test Method | Characterization | Measurement
Status
  • MAY 2020 Published
ABSTRACT

This document describes a protocol for assessing the effect of a nanoparticle formulation on the basic immunologic function of human lymphocytes, i.e. measurement of lymphocyteproliferative responses. This assay will allow for measurement of a nanoparticles’ ability to suppress leukocyte proliferation induced by phytohemaglutinin (PHA-M). This protocol is a modification of part B of the NCL ITA-6.1 (formerly NCL ITA-6).