Enzyme-Linked Immunosorbent Assay (ELISA) for Detection of Human IL-8 in Culture Supernatants
| Standard Number | ITA-22 |
|---|---|
| Organization |
Nanotechnology Characterization Laboratory
|
| Level | National |
| Category | Test Method | Characterization | Measurement |
| Status |
|
ABSTRACT
CXCR8/IL-8 is a proinflammatory chemokine produced by various types of cells in response to inflammatory stimulus, e.g. bacterial lipopolysaccharide LPS. This document describes experimental procedure for analysis of cell culture supernatants for the presence of IL-8 by enzyme linked immunosorbent assay (ELISA).