Enzyme-Linked Immunosorbent Assay (ELISA) for Detection of Human IL-1β in Culture Supernatants
| Standard Number | ITA-23 |
|---|---|
| Organization |
Nanotechnology Characterization Laboratory
|
| Level | National |
| Category | Test Method | Characterization | Measurement |
| Status |
|
ABSTRACT
ΙL−1β is a pro-inflammatory cytokine produced by various types of cells in response to inflammatory stimulus, e.g. bacterial lipopolysaccharide LPS. This document describes experimental procedure for analysis of culture supernatants by ELISA to detect presence ofIL-1β. NCL protocol ITA-10 should be referred to for details of preparation of culture supernatants.