Enzyme-Linked Immunosorbent Assay (ELISA) for Detection of Human TNF-α in Culture Supernatants
| Standard Number | ITA-24 |
|---|---|
| Organization |
Nanotechnology Characterization Laboratory
|
| Level | National |
| Category | Test Method | Characterization | Measurement |
| Status |
|
ABSTRACT
TNF-α is a proinflammatory cytokine produced by various types of cells in response to inflammatory stimulus, e.g. bacterial lipopolysaccharide LPS. This document describes experimental procedure for analysis of culture supernatants by ELISA to detect presence ofTNF-α. NCL protocol ITA-10 should be referred to for details of preparation of culture supernatants.