Researchers develop new interferometric single-molecule localization microscopy

Various image-based central position estimation (termed "centroid fitting") methods such as 2-D Gaussian fitting methods have been commonly used in single-molecule localization microscopy (SMLM) to precisely determine the location of each fluorophore. Yet it is still a challenge to improve the single-molecule lateral localization precision to molecular scale (< 2 nm) for high throughput nanostructure imaging.