Multivariate patterning of human pluripotent cells reveals induced paracrine factors in kidney organoid development

Bioengineers have shown great promise in creating complex multicellular kidney organoids (tiny, self-organized tissues) in the lab using pluripotent stem cells . They can further improve the procedure for different outcomes, including patterning and maturation of specific cell types, although such experiments are limited by standard tissue culture approaches. Now writing in Science Advances, Nick R. Glass and an interdisciplinary research team at the Australian Institute for Bioengineering and Nanotechnology, Murdoch Children's Research Institute and the Department of Pediatrics and Biomedical Manufacturing in Australia detailed a new full factorial microbioreactor array-based method to perform complex differentiation in the lab.