Imaging Technology Enables Superresolution Inside Whole Cells

Purdue University researchers have developed a technology that enables 3D superresolution imaging inside whole-cell or tissue specimens. The technology allows scientists to locate the positions of biomolecules inside whole cells and tissues with a precision down to a few nanometers. Although superresolution fluorescence microscopy can improve spatial resolution up to tenfold, resolution can be restricted when light signals, emitted from molecules inside a specimen, travel through different parts of cell or tissue structures at different speeds and cause aberrations in the lightwaves. “Our technology allows us to measure wavefront distortions induced by the specimen, either a cell or a tissue, directly from the signals...