Nanotechnology Now - Press Release: An alternative to MINFLUX that enables nanometre resolution in a confocal microscope

Fluorescence microscopy is a major workhorse in life sciences, biophysics, and physical chemistry, as it allows visualization with great specificity and sensitivity. In particular, the detection of single fluorescent molecules has been used to provide information beyond ensemble averages with applications in different fields of research. For example, super-resolution methods based on the localization of single emitters have been used in the last 15 years to study biological systems with unprecedented spatial resolution in the 10-50 nm range, and even enabling the discovery of supramolecular cellular structures. On the other hand, single-molecule tracking has enabled to study individual trajectories of relevant targets that would be otherwise hidden in the average behaviour of an ensemble of unsynchronized molecules.