Nanotechnology – CM-H2DCF-DA assay to assess nanoparticle-induced intracellular reactive oxygen species (ROS) production in macrophage cell line RAW 264.7

Nanotechnology – CM-H2DCF-DA assay to assess nanoparticle-induced intracellular reactive oxygen species (ROS) production in macrophage cell line RAW 264.7

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Standard Number DS/ISO/TS 19006:2016
Organization Danish Standards Denmark
Level National
Category Test Method | Characterization | Measurement
Status
  • SEP 2016 Published
ABSTRACT

ISO/TS 19006:2016 describes how to test and evaluate results obtained from in vitro ROS generation in RAW 264.7 macrophage cells exposed to nano-objects, nanoparticles, their aggregates and agglomerates using the CM-H2DCFDA assay. The protocol in ISO/TS 19006:2016 is limited to use of a 24 well plate so if other plates were to be used, volumes would need to be adjusted and the protocol steps validated to ensure confidence in the test results.