Nanotechnologies — 5-(And 6)-Chloromethyl-2’,7’ Dichloro-Dihydrofluorescein Diacetate (CM-H2DCF-DA) Assay For Evaluating Nanoparticle-Induced Intracellular Reactive Oxygen Species (ROS) Production In RAW 264.7 Macrophage Cell Line

Nanotechnologies — 5-(And 6)-Chloromethyl-2’,7’ Dichloro-Dihydrofluorescein Diacetate (CM-H2DCF-DA) Assay For Evaluating Nanoparticle-Induced Intracellular Reactive Oxygen Species (ROS) Production In RAW 264.7 Macrophage Cell Line

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Standard Number GSO ISO/TS 19006:2021
Organization GCC Standardization Organization World
Level International
Category Test Method | Characterization | Measurement
Status
  • JUL 2021 Published
ABSTRACT

ISO/TS 19006:2016 describes how to test and evaluate results obtained from in vitro ROS generation in RAW 264.7 macrophage cells exposed to nano-objects, nanoparticles, their aggregates and agglomerates using the CM-H2DCFDA assay.

The protocol in ISO/TS 19006:2016 is limited to use of a 24 well plate so if other plates were to be used, volumes would need to be adjusted and the protocol steps validated to ensure confidence in the test results.