Nanotechnology — Toxicity evaluation of nanomaterials using zebrafish embryos with their egg membranes removed

This standard specifies methods to rapidly assess nanomaterial toxicity (early fish, from 0 to 120 hours after fertilization). Information is provided on the importance of acellular egg membrane removal, egg membrane removal procedures, and toxicity evaluation methods for nanomaterials using zebrafish embryos from which egg membranes have been removed. This standard focuses on assessing the toxicity of nanomaterials.