This standard specifies in vitro and in vivo toxicology and ecotoxicological screening test methods suitable for nanomaterials. These methods contribute to the screening of nanomaterials toxicology including ecotoxicology. The toxicology screening test in this guidance technical document can be used for early determination of research and product development, rapid feedback on its potential toxicology/safety risks, or preliminary evaluation. This guidance technical document can be divided into toxicological screening methods related to human health and environment related. Screening tests are relatively simple and inexpensive methods that are easy to perform and provide corresponding indications of whether nanomaterials have potential negative effects on humans and the environment. This guiding technical document focuses on describing the preliminary screening methods applicable to toxicological evaluation. It is a supplement to other foreign standards and is compatible with other international organizations.