This Standard specifies the application of the Limulus test evaluation of nanomaterials, for in vitro biological assay system cells. This Standard applies to be an aqueous medium (for example: water, serum or reaction medium) or extracts dispersed nanomaterials, making nanomaterials with such culture medium at an appropriate time 37��C. This Standard is limited to in vitro test sample, the method can also be applied to animal by the parenteral route of administration of nanomaterials.
