This work will cover to a standard method for generation of silver nanoparticles to be tested for inhalation toxicity on experimental animals. More detailed defining of the scope will be discussed in the following specific items. 1) Generator set-up: flat-plate ceramic heater was connected to an AC power supply and housed within a quartz case 2) Heater dimension: 50 × 5 × 1.5 mm3. 3) Surface temperature: about 1500? 4) Voltage : 85 V 5) Highest temperature on the local heating area, Tmax,: 1140? 6) air changes per hour in an exposure chamber: 12-15 times 7) loading mass of the silver lump : 160 mg 8) Nanoparticle range: 4.5-157 nm 9) Geometric mean diameters of silver nanoparticles: less than 100 nm in nonaggregated/ nonagglomerated form 10) Silver Nanoparticle measurement: SMPS (scanning mobility particle sizer) and CPC (Condensation particle counter), TEM (Transmission Electron microscopy).
