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MIT engineers have designed a new nanoparticle sensor that could enable early diagnosis of cancer with a simple urine test. The sensors, which can detect many different cancerous proteins, could also be used to distinguish the type of a tumor or how it is responding to treatment.
Multiplexed DNA-encoded synthetic urine biomarkers for portable monitoring of invasive colorectal cancer. a, Multiplexed DNA-SUBs are comprised of a polymeric nanocarrier (eight-arm PEG) functionalized with protease-activated peptides barcoded with oligonucleotides. b, Left: Timeline of longitudinal tumor monitoring with DNA-SUB. D, day. Right: histological lung staining of BALB/c mice bearing CRC lung tumors at 11 days (upper) and 21 days (lower) after tumor inoculation. Scale bar, 200 µm. c, Left: Schematic of the fluorogenic assay to identify peptide substrates specifically cleaved by lung tissue homogenates collected 21 days after tumor inoculation. Middle: Peptide cleavage by CRC-bearing and healthy lung tissue homogenates were monitored and cleavage rates normalized to healthy tissue are shown in bar graphs (n = 5 mice per group; mean ± s.e.m.; unpaired two-tailed t-test with Welch’s correction, ****P
Multiplexed DNA-encoded synthetic urine biomarkers for portable monitoring of invasive colorectal cancer. a, Multiplexed DNA-SUBs are comprised of a polymeric nanocarrier (eight-arm PEG) functionalized with protease-activated peptides barcoded with oligonucleotides. b, Left: Timeline of longitudinal tumor monitoring with DNA-SUB. D, day. Right: histological lung staining of BALB/c mice bearing CRC lung tumors at 11 days (upper) and 21 days (lower) after tumor inoculation. Scale bar, 200 µm. c, Left: Schematic of the fluorogenic assay to identify peptide substrates specifically cleaved by lung tissue homogenates collected 21 days after tumor inoculation. Middle: Peptide cleavage by CRC-bearing and healthy lung tissue homogenates were monitored and cleavage rates normalized to healthy tissue are shown in bar graphs (n = 5 mice per group; mean ± s.e.m.; unpaired two-tailed t-test with Welch’s correction, ****P 