Mouse Granulocyte-Macrophage Colony-Forming Unit Assay

Mouse Granulocyte-Macrophage Colony-Forming Unit Assay

Standard Number ITA-3
Organization Nanotechnology Characterization Laboratory USA
Level National
Category Specification
Status
  • JAN 2009 Published
ABSTRACT
Myelosuppression is a common dose limiting toxicity of cytotoxic oncology drugs. Nanoparticles may distribute to bone marrow and/or release drug that distributes to bone marrow. Therefore, understanding potential toxicity of nanoparticles or drugs which nanoparticles carry is an important step in preclinical safety evaluation. This document describes a protocol for quantitative analysis of granulocyte-macrophagecolony-forming units, employing murine bone marrow (BM).