Hep G2 Hepatocyte Glutathione Assay

Hep G2 Hepatocyte Glutathione Assay

Standard Number GTA-3
Organization Nanotechnology Characterization Laboratory USA
Level National
Category Specification
Status
  • JAN 2010 Published
ABSTRACT
This protocol describes the analysis of human hepatocarcinoma cells (Hep G2) for reduced and oxidized glutathione, following treatment with nanoparticle formulations, as part of the NCL in vitro preclinical characterization cascade. A shift from reduced to oxidized glutathione isindicative of oxidative stress, while a decrease in the overall reduced glutathione pool is indicative of conjugative metabolism or impaired synthesis (1). This protocol utilizes a colorimetric method for glutathione determination.