Hep G2 Hepatocarcinoma Cytotoxicity Assay

Standard Number GTA-2
Organization Nanotechnology Characterization Laboratory USA
Level National
Category Specification
  • JAN 2010 Published
This protocol describes the cytotoxicity testing of nanoparticle formulations in human hepatocarcinoma cells (Hep G2), as part of the in vitro NCL preclinical characterization cascade. The protocol utilizes two methods for estimation of cytotoxicity, 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) reduction and lactate dehydrogenase (LDH) release(1, 2).