Autophagic Dysfunction Assay: Qualitative Analysis of MAP LC3 - I to LC3 - II C onversion by Western Blot

Autophagic Dysfunction Assay: Qualitative Analysis of MAP LC3 - I to LC3 - II C onversion by Western Blot

Standard Number GTA-11
Organization Nanotechnology Characterization Laboratory USA
Level National
Category Specification
Status
  • JAN 2013 Published
ABSTRACT
Lysosomal dysfunction is recognized as a potential toxic mechanism for xenobiotics that can result in various pathological states (1). There is concern that nanopaticles, in particular, may cause lysosomal pathologies, since they are likely to accumulate within lysosomes (2). Lysosomal dysfunction could potentially result from nanoparticle biopersistance, or inhibition of lysosomal enzymes, such as inhibition of phospholipase resulting in phospholipidosis, or inhibition of lysosomal protein degradation resulting in lysosomal overload