Enzyme-Linked Immunosorbent Assay (ELISA) for Detection of Human TNF-α in Culture Supernatants

Enzyme-Linked Immunosorbent Assay (ELISA) for Detection of Human TNF-α in Culture Supernatants

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Standard Number ITA-24
Organization Nanotechnology Characterization Laboratory USA
Level National
Category Test Method | Characterization | Measurement
Status
  • JUN 2020 Published
ABSTRACT
TNF-α is a proinflammatory cytokine produced by various types of cells in response to inflammatory stimulus, e.g. bacterial lipopolysaccharide LPS. This document describes experimental procedure for analysis of culture supernatants by ELISA to detect presence ofTNF-α. NCL protocol ITA-10 should be referred to for details of preparation of culture supernatants.