|Organization||Nanotechnology Characterization Laboratory|
|Category||Test Method | Characterization | Measurement|
This panel includes antibody-fluorophore conjugates that allow for analysis of different lymphocyte populations including B cells and T cells (CD8+ T cells, CD4+ T cells, regulatory T (Treg) cells, naïve T cells, and γδ TCR T cells). This panel also determines cellular CD25 and CD154 expression which are markers of proliferation and co-stimulation/presentation, respectively.
When used in conjunction with other immunoassays, this protocol aids in establishing efficacy and safety profiles of engineered nanoparticles used for vaccine or drug delivery. This protocol has two parts, ITA-37.1 described in this document and intended for instrument calibration, and ITA-37.2, described in a separate document and intended for the analysis of nanoparticle-treated cells.