Immunophenotyping: Analysis of Nanoparticle Effects on the Composition and Activation Status of Human Peripheral Blood Mononuclear Cells

Immunophenotyping: Analysis of Nanoparticle Effects on the Composition and Activation Status of Human Peripheral Blood Mononuclear Cells

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Standard Number ITA-37.2
Organization Nanotechnology Characterization Laboratory USA
Level National
Category Test Method | Characterization | Measurement
Status
  • OCT 2022 Published
ABSTRACT

This panel includes antibody-fluorophore conjugates that allow for analysis of different cell populations including CD14+ monocytes, DCs (plasmocytoid (p) and myeloid (m) DCs), and NK cells along with NK T cells. This panel also examines cellular CD69 and CD54 expression which are markers of early activation and adhesion, respectively.

When used in conjunction with other immunoassays, the protocol aids in establishing efficacy and safety profiles of engineered nanoparticles used for vaccine or drug delivery. The protocol has two parts, ITA-37.1 described in a separate document and intended for instrument calibration, and ITA-37.2, described herein and intended for the analysis of nanoparticles-treated cells.